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Updated: Nov 8, 2025
Establishment of a High-throughput Setup for Screening Small Molecules That Modulate c-di-GMP Signaling in Pseudomonas aeruginosa
Published on: June 30, 2016
A Selective Culture Medium for Screening Carbapenem Resistance in Pseudomonas spp
Patrice Nordmann1,2,3,4, Claudine Fournier1, Laurent Poirel1,2,3
1Medical and Molecular Microbiology, Department of Medicine, Faculty of Science and Medicine, University of Fribourg, Fribourg, Switzerland.
Abstract:
The SuperCP medium containing meropenem (2 mg/L) was evaluated for screening of carbapenem-resistant Pseudomonas species. It was evaluated using 29 meropenem-susceptible and 56 meropenem-nonsusceptible Pseudomonas-like clinical isolates, the latter exhibiting a variety of carbapenem-resistance mechanisms. Its sensitivity and specificity of detection were found to be 91% and 100%, respectively. By testing spiked stools, an excellent performance of the medium was also observed for detection of carbapenem-resistant Pseudomonas aeruginosa, with a lowest detection limit ranging from 100 to 102 CFU/mL. This screening medium provides the opportunity to select carbapenem-resistant Pseudomonas and Pseudomonas-related isolates regardless of their resistance mechanism.
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