Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Longitudinal changes in the phenotypic profile of circulating extracellular vesicles in healthy individuals.

Frontiers in cell and developmental biology·2026

Same author

Exploratory NMR-based metabolomics reveals transient lipoprotein changes during prolonged computer gaming.

Metabolomics : Official journal of the Metabolomic Society·2026

Same author

Dynamic changes in body fat distribution, ectopic fat, and related metabolic improvement in response to weigt loss in obesity.

Scientific reports·2026

Same author

Safety of [<sup>68</sup>Ga]Ga-FAPI-46 PET/CT including hemodynamic measurements and patient-reported outcomes in cancer patients.

Clinical physiology and functional imaging·2026

Same author

Metformin increases glycolysis and the stress-induced cytokine GDF15 but not FGF21 in humans.

Frontiers in endocrinology·2026

Same author

Circulating Liver-Derived and CD36<sup>+</sup> Extracellular Vesicles Correlate With Ectopic Liver Fat and Markers of Fibrosis in Metabolic Dysfunction-Associated Steatotic Liver Disease and Decrease During Weight Loss Intervention.

Journal of extracellular vesicles·2026

Same journal

When hemolysis changes interpretation: decision-level shifts and reportability failures in routine coagulation testing.

Scandinavian journal of clinical and laboratory investigation·2026

Same journal

Vitamin K-dependent proteins in patients undergoing head and neck free flap surgery - a prospective observational study.

Scandinavian journal of clinical and laboratory investigation·2026

Same journal

Challenges in the interpretation of amphetamine results: differences of reactivity between immunoassay and immunochromatography.

Scandinavian journal of clinical and laboratory investigation·2026

Same journal

Maternal whole blood mercury, cadmium and lead concentrations during pregnancy and lactation and infant heavy metal status at six months.

Scandinavian journal of clinical and laboratory investigation·2026

Same journal

Performance of Sysmex SP-50 automated slidemakers and stainers in three hematology laboratories.

Scandinavian journal of clinical and laboratory investigation·2026

Same journal

Digital misinformation and parental resistance to newborn heel-prick screening: an Instagram content analysis.

Scandinavian journal of clinical and laboratory investigation·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Nov 8, 2025

The Lactate Dehydrogenase Sequestration Assay — A Simple and Reliable Method to Determine Bulk Autophagic Sequestration Activity in Mammalian Cells

The Lactate Dehydrogenase Sequestration Assay — A Simple and Reliable Method to Determine Bulk Autophagic Sequestration Activity in Mammalian Cells

Published on: July 27, 2018

Fully automatic d-lactate assay using a modified commercially available method.

Rikke Wehner Rasmussen¹, David Straarup², Ole Thorlacius-Ussing^2,3

¹Department of Clinical Biochemistry, Aalborg University Hospital, Aalborg, Denmark.

Scandinavian Journal of Clinical and Laboratory Investigation

|April 21, 2021

Summary

This summary is machine-generated.

Early diagnosis of intestinal ischemia is crucial. This study implements d-lactate analysis in routine settings, providing a rapid 9-minute test for early detection of this life-threatening condition.

Keywords:

Intestines biomarkers d-lactate dehydrogenase early diagnosis fatal outcome infarction ischemia lactate dehydrogenases

More Related Videos

Measurement and Analysis of Extracellular Acid Production to Determine Glycolytic Rate

Measurement and Analysis of Extracellular Acid Production to Determine Glycolytic Rate

Published on: December 12, 2015

Mapping Metabolism: Monitoring Lactate Dehydrogenase Activity Directly in Tissue

Mapping Metabolism: Monitoring Lactate Dehydrogenase Activity Directly in Tissue

Published on: June 21, 2018

Related Experiment Videos

Last Updated: Nov 8, 2025

The Lactate Dehydrogenase Sequestration Assay — A Simple and Reliable Method to Determine Bulk Autophagic Sequestration Activity in Mammalian Cells

The Lactate Dehydrogenase Sequestration Assay — A Simple and Reliable Method to Determine Bulk Autophagic Sequestration Activity in Mammalian Cells

Published on: July 27, 2018

Measurement and Analysis of Extracellular Acid Production to Determine Glycolytic Rate

Measurement and Analysis of Extracellular Acid Production to Determine Glycolytic Rate

Published on: December 12, 2015

Mapping Metabolism: Monitoring Lactate Dehydrogenase Activity Directly in Tissue

Mapping Metabolism: Monitoring Lactate Dehydrogenase Activity Directly in Tissue

Published on: June 21, 2018

Area of Science:

Clinical Chemistry
Biomarker Analysis
Gastrointestinal Physiology

Background:

Intestinal infarction, a critical condition from impaired blood flow, has a high mortality rate.
Early diagnosis and treatment within 6 hours are vital for reducing mortality.
Current biomarkers like d-lactate are not readily available in acute clinical settings.

Purpose of the Study:

To implement and validate a d-lactate analysis method for routine clinical use.
To establish a rapid and reliable diagnostic tool for early detection of intestinal ischemia.
To assess the feasibility of using d-lactate as a point-of-care biomarker.

Main Methods:

A spectrophotometric method utilizing d-lactate dehydrogenase (D-LDH) and nicotinamide-adenine dinucleotide (NAD+) was employed.
Interference from l-lactate was mitigated using pH-inactivation of l-lactate dehydrogenase (L-LDH).
A commercially available d-lactate kit was adapted for automated laboratory equipment, ensuring clinical quality goals were met.

Main Results:

The implemented method demonstrated acceptable analytical performance and a short turnaround time of 9 minutes.
The assay successfully distinguished between the cut-off for intestinal ischemia (around 0.3 mM) and the upper reference limit (0.05 mM).
The setup met all clinical quality goals for d-lactate measurement.

Conclusions:

The developed d-lactate assay is suitable for routine clinical implementation.
This rapid analysis offers potential as a readily available tool for the early diagnosis of intestinal ischemia.
Faster detection of d-lactate can significantly improve patient outcomes by enabling timely intervention.