Microclimate drives intraspecific thermal specialization: conservation perspectives in freshwater habitats

Endemic and relict species are often confined to ecological refugia or over fragmented distributions, representing priority conservation subjects. Within these sites, the individual population may realize distinct niches to a varying degree of specialization. An emblematic example is provided by freshwater species segregated in thermal-mineral springs, where individuals may face highly diverse microclimates in limited geographic areas. Downscaling the characterization of physiological traits to microclimatic niches becomes pivotal to adopt effective conservation measures in these heterogeneous habitats. Melanopsis etrusca (Brot, 1862) is an endangered relict snail endemic to a small number of thermal-mineral streams in central Italy. Here we describe the thermal tolerance of two populations of M. etrusca inhabiting streams with distinctly different thermal regimes, investigating the extent of physiological and behavioural specialization to such diverse microclimatic niches. The comparison of oxygen consumption rates of a population dwelling in temperate streams, characterized by seasonal temperature fluctuations (12-27°C), with a population experiencing constantly hot water (35-38°C) revealed the absence of any seasonal or geographic effect on metabolic compensation. Conversely, mobility performances were maximized in the population inhabiting the hot stream. Interestingly, here, the snails exhibited emersion behaviour outside the water, triggered by temperatures above 37°C. In the field, individuals of this population are observed inactive on stream banks, conceivably to minimize the metabolic cost that otherwise would be induced by remaining in the hot water. Only a few individuals from the temperate stream exhibited the same behaviour when exposed to elevated temperatures, suggesting the exaptation of a pre-existing trait during the evolutionary process of adaptation to hot waters. The present results provide elements for the best practice in future programmes aimed at reintroducing stocks of threatened species across heterogeneous habitats. Our study further underlines the relevance of downscaling data collection for endangered species conservation in order to recognize microclimatic specializations.