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hsa‑miR‑5580‑3p inhibits oral cancer cell viability, proliferation and migration by suppressing LAMC2

Rong Fang1, Qian Lu2, Bo Xu1

  • 1Department of Gastroenterology, Puai Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430033, P.R. China.

Molecular Medicine Reports
|April 21, 2021
PubMed

Insights

MicroRNA‑5580‑3p inhibits oral cancer progression by suppressing LAMC2. This microRNA and LAMC2 show potential as diagnostic biomarkers and therapeutic targets for oral cancer.

Area of Science:

  • Molecular Biology
  • Oncology
  • Biochemistry

Background:

  • Oral cancer (OC) is a significant global health concern.
  • Understanding the molecular mechanisms driving OC progression is crucial for developing effective therapies.
  • The role of microRNAs and their interactions with target genes in OC remains an active area of research.

Purpose of the Study:

  • To investigate the regulatory role of microRNA‑5580‑3p (miR‑5580‑3p) in oral cancer (OC) cell phenotypes.
  • To identify and characterize the interaction between miR‑5580‑3p and laminin subunit γ2 (LAMC2) in OC.
  • To explore the potential of miR‑5580‑3p and LAMC2 as biomarkers and therapeutic targets for OC.

Main Methods:

  • Bioinformatics analysis to predict the miR‑5580‑3p/LAMC2 interactome.
  • Reverse transcription-quantitative PCR (RT-qPCR) to assess miR‑5580‑3p and LAMC2 expression.
  • Western blotting to determine LAMC2 protein levels.
  • Cell proliferation (Cell Counting Kit-8, 5-bromo-2'-deoxyuridine) and migration (wound healing) assays.
  • Luciferase reporter assays to confirm the interaction between miR‑5580‑3p and LAMC2 mRNA.

Main Results:

  • miR‑5580‑3p expression was found to be downregulated in OC tissues and cell lines.
  • LAMC2 expression was significantly upregulated in OC tissues and cell lines.
  • miR‑5580‑3p inhibited OC cell viability, proliferation, and migration.
  • This inhibition was mediated by the suppression of LAMC2 expression by miR‑5580‑3p.

Conclusions:

  • miR‑5580‑3p plays a suppressive role in oral cancer progression.
  • The miR‑5580‑3p/LAMC2 axis is a novel molecular pathway implicated in OC.
  • miR‑5580‑3p and LAMC2 represent promising biomarkers and therapeutic targets for oral cancer diagnosis and treatment.
Keywords:
miRNA‑5580‑3pLAMC2oral cancermigrationinvasion

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