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In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA
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DNA-derived nanostructures selectively capture gram-positive bacteria.

Chan-Jin Kim1, Zhangyong Si1, Sheethal Reghu1

  • 1Centre for Antimicrobial Bioengineering, School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive, Nanyang, 637459, Singapore.

Drug Delivery and Translational Research
|April 21, 2021
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Summary

DNA-based polymeric micelles efficiently target Gram-positive bacteria via their dense DNA corona. Thymine-rich structures showed superior targeting, suggesting potential for bacteria concentration and therapeutic delivery.

Keywords:
Bacteria capturingBacteria targetingDNA block copolymerMagnetic nanoparticlesPeptidoglycan

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Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Microbiology

Background:

  • DNA-based nanostructures offer unique properties for biological applications.
  • Polymeric micelles can encapsulate various payloads and exhibit tunable surface characteristics.
  • Selective targeting of bacteria is crucial for diagnostics and therapeutics.

Purpose of the Study:

  • To demonstrate the bacteria targeting capabilities of DNA-based polymeric micelles.
  • To investigate the role of DNA corona density and sequence in Gram-selective bacterial targeting.
  • To explore the potential of these micelles for bacterial capture and concentration.

Main Methods:

  • Fabrication of DNA-polystyrene (DNA-b-PS) block copolymer micelles.
  • Evaluation of micelle targeting efficiency against Gram-positive and Gram-negative bacteria.
  • Synthesis of DNA nanostructures rich in specific bases (A, T, C, G) to study sequence effects.
  • Incorporation of magnetic nanoparticles (MNPs) for bacteria capture and concentration.

Main Results:

  • DNA-b-PS micelles demonstrated efficient targeting of Gram-positive over Gram-negative bacteria.
  • Single-stranded DNA structures showed significantly lower selectivity.
  • Targeting was attributed to interactions between the DNA corona and bacterial peptidoglycan layers.
  • Thymine-rich (T-rich) micelles exhibited the highest targeting efficiency.
  • Micelles with MNPs successfully captured and concentrated Gram-positive bacteria.

Conclusions:

  • High-density DNA corona on polymeric micelles enables efficient Gram-positive bacteria targeting.
  • DNA sequence, particularly thymine richness, influences targeting specificity.
  • These DNA nanostructures show promise for bacterial concentration and as platforms for targeted therapeutic delivery.