Realization of noncontact confocal optical microsphere imaging microscope
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Updated: Nov 8, 2025

Microtensiometer for Confocal Microscopy Visualization of Dynamic Interfaces
Published on: September 9, 2022
1Department of Electrical and Computer Engineering, National University of Singapore, Singapore, Singapore.
Abstract:
A novel noncontact confocal optical microsphere imaging microscope (COMIM) is proposed to achieve high contrast optical nano-imaging at a high scanning speed. The developed setup obtains images with significantly higher contrast when compared with conventional bright-field microsphere nano-imaging, as well as with a commercial confocal laser scanning microscope (CLSM). With the image-by-image scanning scheme, COMIM takes only 11% time to complete a three-dimensional (3D) scanning image than using a commercial CLSM, while still offers an outstanding resolving power for the nano-features on the sample.
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