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Updated: Nov 8, 2025
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The Multi-organ Chip - A Microfluidic Platform for Long-term Multi-tissue Coculture
Published on: April 28, 2015
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High-throughput organ-on-chip platform with integrated programmable fluid flow and real-time sensing for complex
H Azizgolshani1, J R Coppeta1, E M Vedula1
1Draper, 555 Technology Square, Cambridge, MA 02139, USA. jcharest@draper.com.
Lab on a Chip
|April 21, 2021
Summary
This study introduces a novel organ-on-chip platform that enhances drug development by providing predictive, human-relevant in vitro models. The high-throughput system supports various tissue types and advanced data collection, improving in vitro drug screening accuracy.
Area of Science:
- Biotechnology
- Drug Discovery
- In Vitro Modeling
Background:
- Current drug development lacks predictive human-relevant in vitro models.
- Organ-on-chip (OOC) technology offers a path to physiologically appropriate human tissue models.
- Existing OOC technologies often lack throughput, standard form factors, and data collection compatibility.
Purpose of the Study:
- To present a novel OOC platform with advanced culture capabilities for multiple human tissue models.
- To demonstrate the platform's compatibility with industry-standard formats and high-throughput data collection.
- To enhance the predictive accuracy of in vitro drug screening models.
Main Methods:
- Developed a 96-device OOC platform compatible with industry-standard plates and high-throughput tools.
- Implemented programmable flow control for perfusion and high-shear stress regimes.
- Integrated electrical and oxygen sensors for real-time tissue function quantification.
- Utilized high content screening (HCS) and RNA sequencing (RNA-seq) for comprehensive analysis.
Main Results:
- Demonstrated enhanced hepatic tissue function with perfusion flow and aligned endothelial monolayers with high-shear stress.
- Quantified gut tissue barrier function in real-time using electrical sensors.
- Measured renal active transport and oxygen consumption using optical access and integrated sensors.
- Successfully screened all 96 devices and evaluated gene expression via RNA-seq.
Conclusions:
- The novel OOC platform integrates advanced culture, sensing, and high-throughput screening capabilities.
- This integrated approach accelerates the optimization of in vitro models.
- The platform significantly increases the predictive accuracy of in vitro drug screening.
