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Updated: Nov 8, 2025
Whole Genome Sequencing of Candida glabrata for Detection of Markers of Antifungal Drug Resistance
Published on: December 28, 2017
Two adjacent mutations in the conserved domain of SdhB confer various resistance phenotypes to fluopyram in
Yanxia Shi1, Fadi Zhu1, Bingxue Sun1
1The Key Laboratory of Vegetable Disease Control, Insititute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China.
Background:
For Corynespora cassiicola (Berk. & M.A. Curtis) C.T. Wei, a necrotrophic pathogen with a broad host range and a worldwide distribution, resistance to fluopyram has been attributed to mutations in SdhB/C/D subunit of the succinate dehydrogenase (SDH) complex. In our previous study, two point mutations in SdhB from isoleucine to valine at position 280 (I280V) and histidine to tyrosine at position 278 (H278Y) showed different resistance phenotypes to fluopyram and boscalid. This research was conducted to explore the correlation between the mutation of SdhB-I280V or SdhB-H278Y and resistance to fluopyram or boscalid and its effect on the fitness characteristics of C. cassiicola.
Results:
The sdhB gene in a succinate dehydrogenase inhibitor (SDHI)-sensitive C. cassiicola strain (wild type) was successfully replaced with the mutant sdhB gene (GTT at position 280, SdhB-I280V) or with the mutant sdhB gene (TAC at position 278, SdhB-H278Y,). Compared with the wild-type strain, the replacement mutants exhibited significantly different resistance phenotypes, with SdhB-V280 demonstrating moderate resistance to fluopyram and low resistance to boscalid, while SdhB-Y278 was supersensitive to fluopyram and very highly resistant to boscalid. Both of the mutants exhibited decreased sensitivity to salt stress and increased SDH activity. These two mutations had no effect on the mycelial growth rate, sporulation ability, pathogenicity in vivo, sensitivity to osmotic stress and oxidative stress, cell wall damaging agents, or SHAM.
Conclusion:
Two adjacent mutations in the SdhB gene conferred different resistance phenotypes to SDHIs in C. cassiicola, which is important for the development of alternative antifungal fungicides and fluopyram resistance management. © 2021 Society of Chemical Industry.
