Related Experiment Video
Updated: Nov 8, 2025

Gene Expression Analyses in Human Follicles
Published on: February 17, 2023
Folliculogenesis-related genes are differently expressed in secondary and tertiary ovarian follicles
L B S Moura1, D M Magalhães-Padilha1, A N P Morais2
1Postgraduate Biotechnology, Potiguar University, Natal, RN, Brazil.
Abstract:
The relative mRNA abundance of 10 genes associated with folliculogenesis was compared between late preantral (secondary) and early antral (tertiary) ovarian follicles in goats. In total, 100 follicles in each category were mechanically isolated. The relative transcript abundance of the mRNAs were determined by qPCR. Data were analyzed using unpaired Student's t-test. Of the 10 tested genes, ABLIM mRNA was not detected in either follicle category, six genes (SLIT3, TYMS, GTPBP1, AKR1C4, PIK3R6, and MAOB) were upregulated in secondary follicles compared with tertiary follicles, and three genes (ARHGEF12, CLEC6A, and CYTL1) showed similar mRNA abundances in both secondary and tertiary follicles. In conclusion, SLIT3, GTPBP1, AKR1C4, and PIK3R6 mRNA abundance was upregulated in secondary follicles (preantral phase) compared with in tertiary follicles (antral phase) in goats.
Related Concept Videos
Folliculogenesis
Oogenesis
Each primary oocyte is surrounded by a layer of pre-granulosa cells, forming what is...
Oogenesis
Ovarian Cycle
Hormonal Control of the Ovarian Cycle
Before puberty, the hypothalamus releases GnRH in a low frequency, low amplitude pulsatile manner. This along with the immature hypothalamic-pituitary-gonadal axis activity, results in low estrogen levels and the absence of a fully functional ovarian cycle. At puberty, GnRH secretion increases in both frequency and...

