Vibrio ziniensis sp. nov., isolated from mangrove sediments

A novel Gram-staining-negative, catalase- and oxidase-positive, facultatively anaerobic and rod-shaped motile bacterial strain, designated as ZWAL4003^T, was isolated from mangrove sediments of the Zini Mangrove Forest, Zhangzhou City, PR China. Phylogenetic analysis based on its 16S rRNA gene sequence indicated that ZWAL4003^T was grouped into a separated branch with Vibrio plantisponsor MSSRF60^T (97.38% nucleotide sequence identity) and Vibrio diazotrophicus NBRC 103148^T (97.27%). The major cellular fatty acids were C_{14 : 0} (12.6%), C_{16 : 0} (17.6%), and summed feature 3 (C_{16 : 1}ω6c /C_{16 : 1} ω7c, 45.6%). Its genome had a length of 4650556 bp with 42.8% DNA G+C content, and contained genes involved in the biosynthesis of bacteriocin, β-lactone, resorcinol, N-acyl amino acid, and arylpolyene. The in silico DNA-DNA hybridization and average nucleotide identity values for whole-genome sequence comparisons between ZWAL4003^T and V. plantisponsor LMG 24470^T were clearly below the thresholds used for the delineation of a novel species. The morphological and chemotaxonomic characteristics and the genotypic data of ZWAL4003^T indicated that it represented a novel species of the genus Vibrio. Its proposed name is Vibrio ziniensis sp. nov., and the type strain is ZWAL4003^T (=KCTC 72971^T=MCCC 1A17474^T).