A comprehensive study on 2D, 3D and solid tumor environment to explore a multifunctional biogenic nanoconjugate.

Area of Science:

• Nanomedicine
• Biotechnology
• Materials Science

Background:

• Nanotechnology offers unique advantages for cancer therapy, particularly in drug delivery and imaging.
• Polysaccharide-based nanoparticles are recognized for their potential in nanoparticle-mediated drug delivery systems.
• These nanoparticles exhibit high drug encapsulation, protection, controlled release, and cellular uptake.

Purpose of the Study:

• To fabricate doxorubicin-loaded carboxymethylated PST001 coated iron oxide nanoparticles (DOX@CM-PST-IONPs).
• To evaluate the efficacy of these nanoconjugates for targeted cancer drug delivery and management.
• To assess their potential in eliminating cancer spheroids and reducing tumor burden.

Main Methods:

• Fabrication of doxorubicin-loaded carboxymethylated PST001 coated iron oxide nanoparticles (DOX@CM-PST-IONPs).
• Evaluation of nanoconjugate biocompatibility and toxicity.
• Assessment of cancer cell internalization in 2-D and 3-D cell culture models.
• Analysis of reactive oxygen species production.

Main Results:

• DOX@CM-PST-IONPs were successfully fabricated for targeted drug delivery.
• The nanoconjugates demonstrated biocompatibility and non-toxicity.
• Efficient internalization of cancer cells was observed in both 2-D and 3-D cultures.
• CM-PIONPs exhibited potential for reactive oxygen species generation.

Conclusions:

• Carboxymethylated PST001 coated iron oxide nanoparticles loaded with doxorubicin represent a promising nanoconjugate for cancer therapy.
• These nanoconjugates are effective in cancer cell internalization and possess the potential to reduce tumor burden.
• The ability to generate reactive oxygen species further enhances their therapeutic potential against neoplasia.