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Related Concept Videos

Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

633
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
633

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A Filter-based Surface Enhanced Raman Spectroscopic Assay for Rapid Detection of Chemical Contaminants
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Rapid uropathogen identification using surface enhanced Raman spectroscopy active filters.

Simon D Dryden1, Salzitsa Anastasova2, Giovanni Satta3

  • 1Department of Surgery and Cancer, Imperial College London, St Mary's Hospital, 10Th Floor, QEQM Wing, London, W2 1NY, UK. Simon.dryden@imperial.ac.uk.

Scientific Reports
|April 23, 2021
PubMed
Summary
This summary is machine-generated.

This study presents a novel diagnostic method for urinary tract infections using gold-coated filters and surface-enhanced Raman spectroscopy (SERS). This approach enables rapid and accurate pathogen identification, improving upon current diagnostic limitations.

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Microbiology

Background:

  • Urinary tract infections (UTIs) are common, causing significant morbidity and societal costs.
  • Current diagnostic methods lack the speed for timely pathogen identification, hindering effective treatment.
  • Surface-enhanced Raman spectroscopy (SERS) offers potential for rapid bacterial classification but requires complex sample preparation.

Purpose of the Study:

  • To develop a simplified SERS-based diagnostic approach for UTIs.
  • To utilize gold-coated membrane filters for simultaneous bacterial capture, concentration, and SERS signal enhancement.
  • To assess the diagnostic accuracy and bacterial classification capabilities of the developed SERS-active filters.

Main Methods:

  • Gold-coated membrane filters with an optimal 50 nm thickness were fabricated.
  • Phantom urine infection samples at clinically relevant concentrations (10^5 CFU/ml) were analyzed.
  • SERS was employed to analyze bacteria captured and concentrated on the gold-coated filters.
  • Diagnostic performance was evaluated by distinguishing infected from uninfected samples and classifying specific bacterial species.

Main Results:

  • The gold-coated filters effectively captured and concentrated bacteria from urine, enhancing SERS signals.
  • An accuracy of 91.1% was achieved in identifying infected versus uninfected urine samples.
  • Classification of three common UTI-causing bacteria (Escherichia coli, Enterococcus faecalis, Klebsiella pneumoniae) was accomplished with 91.6% accuracy.

Conclusions:

  • Gold-coated membrane filters integrated with SERS provide a promising platform for rapid UTI diagnostics.
  • This method simplifies sample preparation, overcoming a key limitation for SERS integration into clinical settings.
  • The developed technique demonstrates high accuracy in both UTI detection and pathogen identification.