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DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

DNA Isolation

DNA Isolation

DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.

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Related Experiment Video

Updated: Nov 8, 2025

Streamlined Purification of Plasmid DNA From Prokaryotic Cultures

Streamlined Purification of Plasmid DNA From Prokaryotic Cultures

Published on: January 5, 2011

High-yield purification of exceptional-quality, single-molecule DNA substrates.

Yue Lu¹, Piero Bianco¹

¹Department of Pharmaceutical Sciences, College of Pharmacy, University of Nebraska Medical Center, Omaha, NE 68198-6025, USA.

Journal of Biological Methods

|April 23, 2021

Summary

This summary is machine-generated.

High-quality DNA and RNA substrates are essential for single-molecule studies. This new, optimized purification method ensures reproducible, high yields of nucleic acid substrates for various applications.

Keywords:

DNA substrate TSKgel DNA-stat column chromatography oligonucleotide single-molecule

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Single-step Purification of Macromolecular Complexes Using RNA Attached to Biotin and a Photo-cleavable Linker

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Last Updated: Nov 8, 2025

Streamlined Purification of Plasmid DNA From Prokaryotic Cultures

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Published on: January 5, 2011

Author Spotlight: Advancing Chromatin Research and Overcoming Limitations with a High-Enrichment Locus-Specific Chromatin Isolation Protocol

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Published on: November 17, 2023

Single-step Purification of Macromolecular Complexes Using RNA Attached to Biotin and a Photo-cleavable Linker

Single-step Purification of Macromolecular Complexes Using RNA Attached to Biotin and a Photo-cleavable Linker

Published on: January 3, 2019

Area of Science:

Biochemistry
Molecular Biology
Biophysics

Background:

High-quality nucleic acid substrates are crucial for single-molecule studies.
Existing purification methods are inconsistent, leading to variable yields.

Purpose of the Study:

To develop an optimized, reproducible method for purifying high-quality DNA and RNA substrates.
To address the inconsistency and variable yields of current purification techniques.

Main Methods:

A straightforward, column-based purification approach was developed.
The method utilizes a non-porous anion exchange resin.
Optimization of each substrate construction step was emphasized.

Main Results:

The optimized method produces high yields of exceptional quality hairpin DNA substrates.
The purification approach is reproducible and suitable for experienced researchers.
The method is adaptable for DNA substrates ranging from 70-15000 bp.

Conclusions:

This optimized method provides a reliable way to obtain high-quality nucleic acid substrates for single-molecule experiments.
The technique is broadly applicable to various DNA substrates used in biophysical studies.
The method enhances the consistency and yield of substrate preparation for researchers.