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Related Concept Videos

Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

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Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
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Electrophoresis: Overview01:20

Electrophoresis: Overview

3.0K
Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
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Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

586
Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
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Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method
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CEToolbox: Specialized calculator for capillary electrophoresis users as an android application.

Yannis Nicolas François1, Michael Biacchi1, Rabah Gahoual2

  • 1Laboratoire de Spectrométrie de Masse des Interactions et des Systèmes (LSMIS) UMR 7140 (Unistra-CNRS), Université de Strasbourg, Strasbourg, France.

Electrophoresis
|April 23, 2021
PubMed
Summary

CEToolbox is a free Android app simplifying capillary electrophoresis (CE) calculations for researchers. It provides essential separation properties, aiding daily analysis and improving efficiency for all CE users.

Keywords:
ConductivityFlow rateMobilitySeparation characteristic calculationViscosity

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Area of Science:

  • Analytical Chemistry
  • Separation Science

Background:

  • Capillary Electrophoresis (CE) is a powerful separation technique for charged and neutral molecules.
  • The use of CE has grown significantly since the late 1980s, increasing the number of users in academic and industrial research.
  • CE users frequently encounter challenges with basic separation property calculations.

Purpose of the Study:

  • To develop a mobile application, CEToolbox, to assist the capillary electrophoresis community.
  • To provide quick and easy access to essential CE calculations for users.
  • To simplify daily tasks for CE practitioners on mobile platforms.

Main Methods:

  • Development of a free Android application named CEToolbox.
  • Implementation of features for calculating injection parameters (volume, proportion, amount, rinsing time, electrical field).
  • Inclusion of calculation modules for buffer properties (viscosity, conductivity), separation flow rates, and electroosmotic/effective mobilities for up to 20 compounds.

Main Results:

  • CEToolbox offers a user-friendly interface for accessing critical CE separation data.
  • The application provides calculations for injected sample volume, total capillary volume, and sample proportion.
  • Advanced calculations include buffer viscosity, conductivity, flow rates, and mobilities for multiple compounds.

Conclusions:

  • CEToolbox simplifies routine calculations for capillary electrophoresis users.
  • The free mobile application enhances accessibility and efficiency for CE analysis.
  • CEToolbox supports a wide range of CE users, irrespective of their specific instrumentation.