Microswimmer Combing: Controlling Interfacial Dynamics for Open-Surface Multifunctional Screening of Small Animals

Image-based screening of multicellular model organisms is critical for both investigating fundamental biology and drug development. Current microfluidic techniques for high-throughput manipulation of small model organisms, although useful, are generally complicated to operate, which impedes their widespread adoption by biology laboratories. To address this challenge, this paper presents an ultrasimple and yet effective approach, "microswimmer combing," to rapidly isolate live small animals on an open-surface array. This approach exploits a dynamic contact line-combing mechanism designed to handle highly active microswimmers. The isolation method is robust, and the device operation is simple for users without a priori experience. The versatile open-surface device enables multiple screening applications, including high-resolution imaging of multicellular organisms, on-demand mutant selection, and multiplexed chemical screening. The simplicity and versatility of this method provide broad access to high-throughput experimentation for biologists and open up new opportunities to study active microswimmers by different scientific communities.