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Updated: Nov 8, 2025

Selecting and Isolating Colonies of Human Induced Pluripotent Stem Cells Reprogrammed from Adult Fibroblasts
Published on: February 20, 2012
Reprogramming of a human induced pluripotent stem cell line from one 48-year-old healthy male donor
Ruiyun Guo1, Weihong Mu2, Xin Liu1
1Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, China.
Abstract:
In this study, skin biopsy was collected from a healthy 48-year old male donor with informed consent, and the fibroblasts were isolated from the dermal explant cultures. Here, a human induced pluripotent stem cell (iPSC) line was derived from the fibroblasts using the reprogramming four Yamanaka factors (Oct3/4, Sox2, Klf4, c-Myc). The generated iPSCs were integration-free, displayed the normal karyotype, expressed pluripotency markers and demonstrated trilineage differentiation potential in vitro. This iPSC model will be useful for investigating physiological processes, drug validation as well as a control in pathological mechanistic studies.
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