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Hsp16.3 of mycobacterium tuberculosis in exosomes as a biomarker of tuberculosis

Can Huang1, Liping Pan2, Xiaoli Shen1

  • 1CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, 215163, People's Republic of China.

European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology
|April 24, 2021
PubMed

Insights

Researchers found that the Mycobacterium tuberculosis (MTB) protein Hsp16.3 is present in exosomes from infected cells and tuberculosis patients, suggesting it could be a novel biomarker for diagnosing tuberculosis.

Area of Science:

  • Biochemistry and Molecular Biology
  • Immunology
  • Microbiology

Background:

  • Exosomes are cell-derived vesicles containing proteins and nucleic acids.
  • Mycobacterium tuberculosis (MTB) produces the small heat shock protein Hsp16.3.
  • Identifying reliable tuberculosis biomarkers is crucial for diagnosis and treatment.

Purpose of the Study:

  • To investigate the presence and distribution of Hsp16.3 protein in exosomes.
  • To evaluate the potential of Hsp16.3 in exosomes as a biomarker for tuberculosis diagnosis.

Main Methods:

  • Overexpression of Hsp16.3 protein in cells.
  • Infection of U937 cells with MTB and analysis of secreted exosomes.
  • Detection of Hsp16.3 protein in exosomes from blood of tuberculosis patients.

Main Results:

  • Overexpressed Hsp16.3 protein was efficiently packaged into exosomes.
  • MTB-infected U937 cells secreted excessive amounts of Hsp16.3 in exosomes.
  • Significant levels of Hsp16.3 protein were found in blood exosomes of tuberculosis patients.

Conclusions:

  • Hsp16.3 protein can be effectively packaged into exosomes.
  • Exosomal Hsp16.3 levels are elevated in MTB infection and tuberculosis.
  • This research indicates a potential for an exosome-based biomarker for tuberculosis diagnosis.
Keywords:
BiomarkerExosomesHsp16.3Mycobacterium tuberculosis

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