Hyperthermophilic composting significantly decreases methane emissions: Insights into the microbial mechanism

Methane (CH₄) emissions from thermophilic composting (TC) are a substantial contributor to climate change. Hyperthermophilic composting (HTC) can influence CH₄-related microbial communities at temperatures up to 80 °C, and thus impact the CH₄ emissions during composting. This work investigated CH₄ emissions in sludge-derived HTC, and explored microbial community succession with quantitative PCR and high-throughput sequencing. Results demonstrated that HTC decreased CH₄ emissions by 52.5% compared with TC. In HTC, the CH₄ production potential and CH₄ oxidation potential were nearly 40% and 64.1% lower than that of TC, respectively. There was a reduction in the quantity of mcrA (3.7 × 10⁸ to 0 g^-1 TS) in HTC, which was more significant than the reduction in pmoA (2.0 × 10⁵ to 2.1 × 10⁴ g^-1 TS), and thus lead to reduce CH₄ emissions. It was found that the abundance of most methanogens and methanotrophs was inhibited in the hyperthermal environment, with a decline in Methanosarcina, Methanosaeta and Methanobrevibacter potentially being responsible for reducing the CH₄ emissions in HTC. This work provides important insight into mitigating CH₄ emissions in composting.