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Radioiodination of proteins and lipoproteins using N-bromosuccinimide as oxidizing agent
H J Sinn1, H H Schrenk, E A Friedrich
1Department of Nuclear Medicine, Deutsches Krebsforschungszentrum, Heidelberg, Federal Republic of Germany.
Analytical Biochemistry
|April 1, 1988
Abstract:
In an attempt to improve conditions for radioiodination of sensitive proteins we used N-bromosuccinimide as a mild oxidizing agent. Under gentle conditions we increased the average labeling efficiency of a wide variety of proteins to above 97%. There was no loss of binding activity of low density lipoprotein particles, which are most sensitive to oxidation. Depending on high labeling efficiency, our method reduces preparation time as well as radioactive waste, costs, and irradiation exposure to personnel.