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Updated: Nov 8, 2025

Estimating Sediment Denitrification Rates Using Cores and N2O Microsensors
Published on: December 6, 2018
Effectively stimulating partial denitrification to utilize dissolved slowly-biodegradable organic matter by
Liangliang Shi1, Xiyao Li1, Qiong Zhang1
1National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, PR China.
Abstract:
In this study, partial denitrification (PD, nitrate → nitrite) using dissolved slowly-biodegradable organic matter (DSBOM) was effectively established by introducing biosorption and hydrolytic acidification (HA) as a pretreatment for carbon capture and conversion. After 119 days of optimized operation, an efficient nitrate to nitrite transformation of 80% was achieved, with an influent nitrate level of 40 mg/L and DSBOM level of 183.8 mg/L. There was a significant shift from exogenous PD to endogenous PD, with energy supplied by HA products of captured DSBOM, i.e., acetate, saccharide and intracellular poly-hydroxyalkanoates (PHAs), jointly facilitating nitrite production. This was well explained by that genera Dechloromonas (26.7%), possibly responsible for carbon HA and nitrite production, were enriched; while abundant enzymes for glycolysis, acetate fermentation and PHAs storage, and 2.6 times more nitrate reductases than nitrite reductases were identified. These results highlight a novel carbon capture reuse and PD-based anammox strategy to cost-effectively treat nitrogen.
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