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Related Experiment Video

Updated: Nov 8, 2025

Continuously-stirred Anaerobic Digester to Convert Organic Wastes into Biogas: System Setup and Basic Operation

Continuously-stirred Anaerobic Digester to Convert Organic Wastes into Biogas: System Setup and Basic Operation

Published on: July 13, 2012

Methane production from syngas using a trickle-bed reactor setup.

Nabin Aryal¹, Mikkel Odde¹, Cecilie Bøgeholdt Petersen¹

¹Department of Biological and Chemical Engineering, Hangøvej 2, DK-8200 Aarhus N, Denmark.

Bioresource Technology

|April 25, 2021

Summary

This summary is machine-generated.

Waste biomass gasification produces syngas that can be converted to biomethane. Supplementing with hydrogen (H2) upgraded the syngas to natural gas quality, achieving high methane (CH4) concentrations.

Keywords:

Biomethane Hydrogen Syngas methanation Trickle-bed reactor Upgrading to natural gas quality

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Last Updated: Nov 8, 2025

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Area of Science:

Biotechnology
Environmental Science
Chemical Engineering

Background:

Syngas, a product of waste biomass gasification, comprises carbon monoxide (CO), carbon dioxide (CO2), and hydrogen (H2).
Syngas can be converted into methane (CH4) for biofuel production, aiming for natural gas quality (biomethane).

Purpose of the Study:

To investigate the methanation and upgrading of syngas to biomethane using manure (AD-M) and sludge (AD-WW) based inocula in a fed-batch trickle-bed reactor.
To assess the impact of exogenous hydrogen (H2) addition on syngas upgrading to biomethane quality.

Main Methods:

Fed-batch trickle-bed reactor system.
Utilized manure (AD-M) and sludge (AD-WW) based inocula for microbial methanation.
Analyzed syngas composition and microbial communities.

Main Results:

Initial methanation resulted in high CO2 concentrations, not meeting biomethane quality standards.
Simultaneous upgrading with exogenous H2 addition achieved high CH4 concentrations: 91.0 ± 3.5% (AD-WW) and 95.3 ± 1.0% (AD-M).
Microbial community analysis revealed differences between AD-M and AD-WW, indicating functional redundancy.

Conclusions:

Exogenous H2 addition is crucial for upgrading syngas to biomethane quality.
Different microbial inocula can achieve functional redundancy in syngas methanation and upgrading.
This process offers a pathway for converting waste biomass into high-quality biomethane.