Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

510
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
510
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.6K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

MLL3 adaptor function, not methyltransferase catalytic activity, is essential for breast tumor suppression.

bioRxiv : the preprint server for biology·2026
Same author

Transcobalamin receptor CD320, responsible for vitamin B<sub>12</sub> cellular uptake, is present on the cell surface as a homo-oligomer.

The Journal of biological chemistry·2026
Same author

Mevalonate pathway rewiring driven by enhancer remodelling confers resistance to KRAS inhibitors in colorectal cancer.

Nature communications·2026
Same author

Frequency-scanning nonlinearity suppression for FSI ranging based on a phenomenological modeling approach.

Optics express·2026
Same author

The sleeping threat: targeting cancer dormancy to transform metastasis therapy.

Nature reviews. Cancer·2026
Same author

Virtual-interferometer approach to frequency-scanning nonlinearity calibration via harmonic extraction from a Fabry-Pérot etalon.

Optics letters·2026
Same journal

Correction to: 'Stokes settling and particle-laden plumes: implications for deep-sea mining and volcanic eruption plumes' (2020), by Mingotti et al.

Philosophical transactions. Series A, Mathematical, physical, and engineering sciences·2026
Same journal

A stable hothouse triggered by a tipping mechanism.

Philosophical transactions. Series A, Mathematical, physical, and engineering sciences·2026
Same journal

Beyond distance: quantifying point cloud dynamics with persistent homology and dynamic optimal transport.

Philosophical transactions. Series A, Mathematical, physical, and engineering sciences·2026
Same journal

Global stability of the Atlantic overturning circulation: edge state, long transients and boundary crisis under CO2 forcing.

Philosophical transactions. Series A, Mathematical, physical, and engineering sciences·2026
Same journal

Morse index classification and landscape of Kuramoto system for Hebbian-based binary pattern recognition.

Philosophical transactions. Series A, Mathematical, physical, and engineering sciences·2026
Same journal

Interpretable and equation-free response theory for complex systems.

Philosophical transactions. Series A, Mathematical, physical, and engineering sciences·2026
See all related articles

Related Experiment Video

Updated: Nov 8, 2025

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

25.6K

GPU-accelerated real-time reconstruction in Python of three-dimensional datasets from structured illumination

Hai Gong1, Wenjun Guo1, Mark A A Neil1

  • 1Department of Physics, Blackett Laboratory, Imperial College, Prince Consort Road, London SW7 2AZ, UK.

Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences
|April 26, 2021
PubMed
Summary
This summary is machine-generated.

We developed a new structured illumination microscopy system for light-sheet imaging. This system achieves super-resolution, exceeding diffraction limits by over two times, and processes images rapidly for live samples.

Keywords:
GPU processingfluorescence microscopylight-sheet microscopyreconstruction algorithmstructured illumination microscopysuper-resolution microscopy

More Related Videos

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects
10:16

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects

Published on: February 8, 2014

12.5K
Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction
12:38

Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction

Published on: August 9, 2011

17.6K

Related Experiment Videos

Last Updated: Nov 8, 2025

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

25.6K
Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects
10:16

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects

Published on: February 8, 2014

12.5K
Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction
12:38

Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction

Published on: August 9, 2011

17.6K

Area of Science:

  • Microscopy
  • Optical Imaging
  • Biophysics

Background:

  • Structured illumination microscopy (SIM) enhances resolution beyond the diffraction limit.
  • Light-sheet microscopy offers advantages for live-cell imaging and large samples.
  • Integrating SIM with light-sheet microscopy presents unique implementation challenges.

Purpose of the Study:

  • To develop and validate a structured illumination microscopy system compatible with light-sheet geometry.
  • To achieve super-resolution imaging with a factor of over 2 improvement in resolution.
  • To implement and optimize reconstruction algorithms for efficient data processing.

Main Methods:

  • A hexagonal illumination pattern generated by interfering three coherent beams.
  • Acquisition of seven laterally shifted pattern images for reconstruction.
  • Development of three data processing methods (groups of seven, stream, batch) for 2D and 3D data.
  • Implementation of GPU-accelerated reconstruction algorithms in Python.

Main Results:

  • Demonstrated super-resolution imaging surpassing the diffraction limit by over 2x in a light-sheet setup.
  • Identified and mitigated axial motion artifacts using a filtering operation in batch processing.
  • Achieved high processing speeds: over 239 raw frames/sec (512x512) and 34 super-resolved frames/sec (1024x1024).

Conclusions:

  • The presented system enables efficient super-resolution light-sheet microscopy.
  • The developed algorithms provide fast and robust image reconstruction, suitable for dynamic samples.
  • This work contributes to advancing super-resolution microscopy techniques for biological research.