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MBNL1 Suppressed Cancer Metastatic of Skin Squamous Cell Carcinoma Via by TIAL1/MYOD1/Caspase-9/3 Signaling Pathways

Jiaorong Chen1, Jiaqi Wang1, Jingyi Qian1

  • 1Department of Anatomy & Embryo-Histology, Basic Medical College, 240515Hubei University of Chinese Medicine, Wuhan, Hubei Province, China.

Technology in Cancer Research & Treatment
|April 26, 2021
PubMed
Abstract

Insights

Muscleblind-like protein 1 (MBNL1) suppresses skin squamous cell carcinoma (SSCC) metastasis by regulating TIAL1/MYOD1/Caspase-3 signaling. MBNL1

Area of Science:

  • Oncology
  • Molecular Biology
  • Dermatology

Background:

  • Skin squamous cell carcinoma (SSCC) incidence is rising globally.
  • SSCC metastasis poses a significant life-threatening risk.
  • Understanding SSCC molecular mechanisms is crucial for effective treatment.

Purpose of the Study:

  • To investigate the role of muscleblind-like protein 1 (MBNL1) in SSCC.
  • To elucidate the underlying molecular mechanism of MBNL1 in SSCC progression.

Main Methods:

  • In vitro study using SCL-1 cells transfected with MBNL1 or siMBNL1.
  • Assessed cell growth and metastasis using MTT assays, LDH activity ELISA, and Transwell migration assays.
  • Analyzed protein expression changes via Western blot, focusing on TIAL1, MyoD1, and Caspase-3.

Main Results:

  • MBNL1 down-regulation enhanced SSCC cell metastasis in vitro.
  • MBNL1 up-regulation reduced SSCC cell metastasis in vitro.
  • MBNL1 influenced the expression of TIAL1, MyoD1, and Caspase-3, with TIAL1/MyoD1 inhibition affecting MBNL1's role.

Conclusions:

  • MBNL1 acts as a suppressor of SSCC metastatic capacity.
  • The TIAL1/MYOD1/Caspase-3 signaling pathway mediates MBNL1's function in SSCC.
  • MBNL1 represents a potential therapeutic target for inhibiting SSCC metastasis.
Keywords:
MBNL1MYOD1TIAL1signaling pathwaysskin squamous cell carcinoma

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