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Updated: Nov 8, 2025
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3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography
Published on: October 24, 2019
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Visualization Ability of Phase-Contrast Synchrotron-Based X-Ray Imaging Using an X-Ray Interferometer in Soft Tissue
Thet-Thet Lwin1,2, Akio Yoneyama3, Hiroko Maruyama1,2
1School of Allied Health Sciences, Kitasato University, Sagamihara, Kanagawa, Japan.
Technology in Cancer Research & Treatment
|April 26, 2021
Summary
Phase-contrast synchrotron X-ray imaging accurately identifies cancer cell characteristics in tumors. This advanced imaging technique offers detailed 3D views for preclinical cancer research.
Area of Science:
- Biomedical Imaging
- Radiology
- Cancer Research
Background:
- Phase-contrast synchrotron X-ray imaging offers high sensitivity and resolution for biological soft tissues.
- It can depict fine morphological structures, including tumors, with great detail.
Purpose of the Study:
- To quantitatively compare phase-contrast synchrotron X-ray computed tomography (CT) with histopathological images of rat testicular tumors.
- To demonstrate the reliability of this imaging technique in identifying cancer cell characteristics.
Main Methods:
- Utilized phase-contrast synchrotron-based X-ray computed tomography (CT).
- Compared CT images with hematoxylin-eosin-stained histopathological sections of rat testicular tumors.
- Analyzed the correlation between absolute densities from CT and nuclear chromatin densities from histology.
Main Results:
- A strong correlation was observed between absolute densities measured on CT images and nuclear chromatin densities in histological images.
- Phase-contrast synchrotron X-ray imaging reliably identified cancer cell characteristics within soft tissue tumors.
- 3D synchrotron-based phase-contrast X-ray CT enabled detailed screening of tumor structures (solid, cystic, fibrous, blood clots) with 26 μm resolution.
Conclusions:
- Phase-contrast synchrotron X-ray imaging using an X-ray interferometer is effective for depicting tumor cell characteristics.
- The technique provides valuable 3D information capabilities for preclinical cancer research.
- This imaging method shows significant potential for advancing the study of tumors.
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