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Updated: Nov 8, 2025
Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors
Published on: February 7, 2018
Fluorogenic Biosensors Constructed via Aggregation-induced Emission Based on Enzyme-catalyzed Coupling Reactions for
Xiaogang Li1,2, Wenxiu Yu3, Hongjie Zhao3
1Medical Science Research Center, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China.
Abstract:
Hydrogen peroxide (H2O2) is a main reactive oxygen by-product produced in the metabolism of organisms and a common biomarker of oxidative stress. Aggregation-induced emission (AIE) probes for H2O2 have been proposed. Such AIEgens mostly use benzeneboronic acid as a recognition group. Recently, a strategy involving enzyme-catalyzed polymerization of AIE compounds shows great potential in AIEgens design. We herein modify the AIE motif, tetraphenylethene (TPE) with o-phenylenediamine (TPE-TAF), which can be oxidated by H2O2 in HRP to form an intramolecular phenazine structure. Compared with a similar approach, the proposed strategy is simple and the TPE-TAF showed a sensitive "turn-on" fluorescence with H2O2. The detection limit (LOD) is 3.39 μM and the probe is highly specific against H2O2. We further verified the reaction mechanism of the enzyme-catalyzed coupling reaction. The probe is a promising candidate as a stable and safe fluorescent substrate in H2O2 sensing.
