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Updated: Nov 8, 2025

Surgical Injury to the Mouse Pancreas through Ligation of the Pancreatic Duct as a Model for Endocrine and Exocrine Reprogramming and Proliferation
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Surgical Injury to the Mouse Pancreas through Ligation of the Pancreatic Duct as a Model for Endocrine and Exocrine Reprogramming and Proliferation

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Reg4 regulates pancreatic regeneration following pancreatitis via modulating the Notch signaling

Juanjuan Dai1,2,3, Yan He1,2, Mingjie Jiang2,4

  • 1Department of Gastroenterology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Journal of Cellular Physiology
|April 26, 2021
PubMed

Insights

Regenerating family member 4 (Reg4) is crucial for pancreatic regeneration after pancreatitis. Reg4 knockdown impairs acinar-to-ductal metaplasia and resolution by regulating Notch activation, highlighting its therapeutic potential.

Area of Science:

  • Gastroenterology and Hepatology
  • Cell Biology
  • Regenerative Medicine

Background:

  • Pancreatic regeneration is vital for restoring exocrine function after acute pancreatitis.
  • Failure in regeneration can lead to severe complications like pancreatic exocrine insufficiency.
  • The molecular regulators and mechanisms governing pancreatic regeneration are not fully understood.

Purpose of the Study:

  • To identify novel regulators of pancreatic regeneration.
  • To elucidate the mechanisms underlying Reg4's role in pancreatic regeneration.
  • To explore Reg4 as a potential therapeutic target for pancreatitis.

Main Methods:

  • Utilized an inducible Tet-on system for gene manipulation.
  • Performed Reg4 knockdown in models of acute pancreatitis.
  • Assessed acinar-to-ductal metaplasia and pancreatitis resolution.
  • Investigated Reg4's effect on Notch signaling pathway activation in vitro and in vivo.

Main Results:

  • Regenerating family member 4 (Reg4) knockdown significantly impaired pancreatic regeneration.
  • Reg4 deficiency affected both acinar-to-ductal metaplasia and the resolution phase of pancreatitis.
  • Reg4 was confirmed to regulate Notch activation, a key pathway in regeneration.
  • Reg4 plays a critical role in the recovery process following pancreatic injury.

Conclusions:

  • Regenerating family member 4 (Reg4) is a newly identified regulator of pancreatic regeneration.
  • Reg4 influences pancreatic recovery by modulating Notch signaling.
  • Reg4 represents a potential therapeutic target for enhancing pancreatic regeneration and treating pancreatitis.
Keywords:
ADMNotchReg4cell proliferationpancreatic regeneration

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