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Comprehensive phylogeny of Konosirus punctatus (Clupeiformes: Clupeidae) based on transcriptomic data
Fangrui Lou1, Shengyao Qiu1, Yongzheng Tang1
1School of Ocean, Yantai University, Yantai 264005, China.
Bioscience Reports
|April 26, 2021
Summary
This study presents the whole transcriptome of Konosirus punctatus, a key marine species. The research provides foundational molecular data and phylogenetic insights for K. punctatus, aiding future biological studies.
Area of Science:
- Marine Biology
- Genomics
- Ecosystem Structure
Background:
- Konosirus punctatus is ecologically significant in marine ecosystems.
- It is a native Northwest Pacific species supporting commercial fisheries.
Purpose of the Study:
- To generate the whole transcriptome of Konosirus punctatus.
- To provide foundational molecular data for K. punctatus research.
- To establish the phylogenetic relationship of K. punctatus.
Main Methods:
- Illumina RNA-seq technology was used to sequence the whole transcriptome from combined tissues.
- De novo assembly was performed to generate unigenes.
- Phylogenetic analysis was conducted using single-copy orthologous genes.
Main Results:
- A total of 46,087,110 clean reads were obtained, assembled into 71,610 unigenes.
- 43,974 unigenes were annotated in at least one database.
- Phylogenetic analysis indicated K. punctatus diverged from related species 61.16-92.52 million years ago.
Conclusions:
- The study successfully generated a comprehensive transcriptome for K. punctatus.
- This provides essential molecular information for future research on this important marine species.
- Phylogenetic analysis clarifies the evolutionary history of K. punctatus.
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