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Related Experiment Video

Updated: Nov 8, 2025

TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis
07:44

TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis

Published on: June 8, 2020

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TMTpro-18plex: The Expanded and Complete Set of TMTpro Reagents for Sample Multiplexing.

Jiaming Li1, Zhenying Cai2,3, Ryan D Bomgarden4

  • 1Department of Cell Biology, Harvard Medical School, Boston 02115, Massachusetts, United States.

Journal of Proteome Research
|April 26, 2021
PubMed
Summary
This summary is machine-generated.

The new TMTpro-18plex reagents enable simultaneous protein profiling of 18 samples, expanding multiplexing capabilities for complex proteomic and phosphoproteomic studies with minimal missing values.

Keywords:
18plexBYL-719KIN-193MCF10APTENTMTproeclipsereal-time search

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Area of Science:

  • Proteomics
  • Mass Spectrometry
  • Biochemistry

Background:

  • The TMTpro-16plex series expanded isobaric tagging reagents, building upon the TMT-11plex.
  • The TMTpro molecule's proline backbone allows for additional isotope combinations.

Purpose of the Study:

  • Introduce TMTpro-134C and TMTpro-135N labeling reagents for 18-plex global protein profiling.
  • Demonstrate the utility of the TMTpro-18plex set for complex experimental designs.

Main Methods:

  • Profiling the proteome and phosphoproteome of isogenic mammary epithelial cell lines using the TMTpro-18plex reagent set.
  • Comparing the TMTpro-18plex data with a TMTpro-16plex experiment.

Main Results:

  • Simultaneous global protein profiling of 18 samples with minimal missing values achieved.
  • High quantitative correlation and similar depth of quantification observed between 18plex and 16plex experiments.
  • Identified changes in protein abundance profiles across different conditions in isogenic cell lines.

Conclusions:

  • TMTpro-18plex reagents significantly expand sample multiplexing capacity in mass spectrometry-based proteomics.
  • Enables more complex and innovative experimental designs for proteomic and phosphoproteomic analyses.
  • Facilitates in-depth investigation of biological conditions with increased sample numbers.