![Quantitative SERS Detection of Uric Acid via Formation of Precise Plasmonic Nanojunctions within Aggregates of Gold Nanoparticles and Cucurbit[n]uril](/_next/image?url=https%3A%2F%2Fcloudfront.jove.com%2FCDNSource%2Fteasers%2F61682.jpg&w=3840&q=50)
Tailoring cysteine detection in colorimetric techniques using Co/Fe-functionalized mesoporous silica nanoparticles.
Morvarid Aghayan1, Ali Mahmoudi1, Mohammad Reza Sazegar1
1Department of Chemistry, Faculty of science, Islamic Azad University, North Tehran Branch, Tehran, Iran. mahmoudiali.ac@gmail.com m_r_sazegar@yahoo.com.
Journal of Materials Chemistry. B
|April 26, 2021
Summary
New mesoporous silica nanoparticles (MSNs) with iron and cobalt show high peroxidase-like activity. This enables sensitive colorimetric detection of hydrogen peroxide (H2O2) and cysteine (Cys) for potential medical diagnostics.
Area of Science:
- Nanomaterials Science
- Biomedical Engineering
- Analytical Chemistry
Background:
- Cysteine (Cys) is vital in physiological functions and disease diagnosis, driving demand for sensitive detection methods.
- Nanomaterial-based peroxidase mimetics are emerging as promising tools for biosensing applications.
- Mesoporous silica nanoparticles (MSNs) offer versatile platforms for incorporating catalytic metal species.
Purpose of the Study:
- To synthesize and characterize novel iron and cobalt-doped mesoporous silica nanoparticles (Co/Fe-MSN).
- To evaluate the peroxidase-like activity of the synthesized Co/Fe-MSN materials.
- To develop a colorimetric sensing platform for hydrogen peroxide (H2O2) and cysteine (Cys) detection.
Main Methods:
- Synthesis of Co/Fe-MSN with varying cobalt content (1%, 3%, 5%).
- Characterization using FTIR, SEM, TEM, XRD, and nitrogen adsorption-desorption.
- Kinetic analysis of peroxidase activity using 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and determination of H2O2 and Cys limits of detection (LOD).
Main Results:
- Co/Fe-MSN (1%) exhibited superior peroxidase-like activity compared to other formulations.
- A colorimetric sensor was successfully developed based on Co/Fe-MSN (1%).
- Achieved low limits of detection: 1.1 μM for H2O2 and 0.89 nM for Cys.
Conclusions:
- The synthesized Co/Fe-MSN (1%) acts as an efficient enzyme mimic with significant peroxidase-like activity.
- The developed colorimetric sensing platform demonstrates high sensitivity and potential for H2O2 and Cys detection.
- These nanomaterials show promise for applications in medical diagnostics and biological system monitoring.
