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Updated: Nov 8, 2025

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Preparation of Intact Tissue for Microscopic Analysis of the Endosperm Cell Layer in Developing and Mature Arabidopsis Seeds
Published on: May 16, 2025
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Arabidopsis thaliana Mature Endosperm Dissection and Isolation of Genomic DNA from Mature Seed Tissues
Mayumi Iwasaki1, Luis Lopez-Molina2,3
1Department of Plant Biology, University of Geneva, Geneva, Switzerland. Mayumi.Iwasaki@unige.ch.
Methods in Molecular Biology (Clifton, N.J.)
|April 26, 2021
Abstract:
We describe methods to separate endosperms and embryos from Arabidopsis thaliana mature seeds in large amounts and to isolate high-quality genomic DNA from those tissues. The resulting materials are suitable for analysis of DNA methylation by bisulfite sequencing or histone modifications by chromatin immunoprecipitation (ChIP).

