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Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

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Published on: April 14, 2015

Fluorescent Convertible Capsule Coding Systems for Individual Cell Labeling and Tracking.

Polina A Demina¹, Olga A Sindeeva^1,2, Anna M Abramova¹

¹Saratov State University, 83 Astrakhanskaya Street, Saratov 410012, Russia.

ACS Applied Materials & Interfaces

|April 26, 2021

Summary

Researchers developed a novel optical tagging system for precisely tracking individual cells. This method uses fluorescently convertible capsules for robust, long-term cell labeling and migration studies in developmental biology.

Keywords:

carbon nanoparticles cell imaging cell tracking encapsulation fluorescent label microcapsules photoconversion

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Area of Science:

Biomedical Science
Developmental Biology
Cell Biology

Background:

Significant interest exists in optical tagging for studying cell behavior and migration.
Current methods lack the ability for on-demand, in situ labeling and discrimination of individual cells.
Long-term tracking of single cells within large populations remains a challenge.

Purpose of the Study:

To demonstrate a novel optical tagging system for individual cell labeling and tracking.
To enable on-demand, in situ marking and discrimination of cells.
To facilitate long-term monitoring of cell migration in complex populations.

Main Methods:

Development of a fluorescently convertible capsule coding system.
Utilizing rhodamine B dye co-confined with carbon nanodots within polyelectrolyte capsules.
Photoconversion technique for on-demand labeling of individual cells.

Main Results:

The developed capsule system demonstrates robust performance.
The capsules are actively uptaken by various cell lines.
The system exhibits low toxicity in cellular environments.
Successful code-like marking and long-term tracking of multiple individual cells were achieved.

Conclusions:

The novel fluorescent convertible capsule system offers a robust solution for individual cell tagging.
This technology enables precise, long-term tracking and migration studies of cells in large populations.
The system holds significant potential for advancing research in developmental biology and biomedical science.