Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

DNA polymerase alpha activity in mitogen-activated lymphocytes.

J Stec1, D Busbee

  • 1Department of Anatomy, College of Veterinary Medicine, Texas A&M University, College Station 77843.

Biochemical and Biophysical Research Communications
|June 30, 1988
PubMed
Summary

Mitogen stimulation activates DNA polymerase alpha (A1) to an A2-like form and induces synthesis of DNA polymerase alpha A2 in lymphocytes, increasing DNA replication activity.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A glyconutritional mixture (Ambrotose®) provides some amelioration to mice with coxsackievirus-induced pancreatitis.

Age·2013
Same author

Protection from glutathione depletion by a glyconutritional mixture of saccharides.

Age·2013
Same author

Alteration of estrogen-regulated gene expression in human cells induced by the agricultural and horticultural herbicide glyphosate.

Human & experimental toxicology·2007
Same author

Alteration of gene expression in human cells treated with the agricultural chemical diazinon: possible interaction in fetal development.

Human & experimental toxicology·2006
Same author

Altered gene expression in human cells treated with the insecticide diazinon: correlation with decreased DNA excision repair capacity.

Human & experimental toxicology·2006
Same author

Bortezomib (VELCADE) in metastatic breast cancer: pharmacodynamics, biological effects, and prediction of clinical benefits.

Annals of oncology : official journal of the European Society for Medical Oncology·2006

Area of Science:

  • Molecular Biology
  • Cellular Biology
  • Biochemistry

Background:

  • DNA polymerase alpha is crucial for DNA replication.
  • Lymphocyte activation involves complex regulatory mechanisms affecting DNA synthesis.

Purpose of the Study:

  • To investigate the differential roles and regulation of DNA polymerase alpha isozymes (A1 and A2) during lymphocyte activation.
  • To elucidate the molecular mechanisms underlying the increase in DNA polymerase alpha activity upon mitogen stimulation.

Main Methods:

  • Isolation and separation of DNA polymerase alpha isozymes from lymphocytes.
  • Enzymatic activity assays and interaction studies with phosphatidylinositol and phosphatidylinositol kinase.
  • Cycloheximide treatment to assess de novo protein synthesis.

Related Experiment Videos

Main Results:

  • Quiescent lymphocytes primarily contain DNA polymerase alpha A1; mitogen-stimulated lymphocytes contain both A1 and A2.
  • DNA polymerase alpha A1, but not A2, can be activated by phosphatidylinositol and phosphatidylinositol kinase.
  • Mitogen stimulation increases total DNA polymerase alpha activity through A1 activation to an A2-like form and de novo synthesis of A2.

Conclusions:

  • Lymphocyte activation involves a dual mechanism for enhancing DNA polymerase alpha activity: post-translational modification of A1 and de novo synthesis of A2.
  • The phosphoinositide pathway plays a role in activating DNA polymerase alpha A1 during lymphocyte proliferation.