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Related Experiment Videos

Quantitative study on creatine metabolism in sheep tissues.

G P Xue1, A M Snoswell, R C Fishlock

  • 1Department of Animal Sciences, Waite Agricultural Research Institute, University of Adelaide, Glen Osmond, South Australia 5064.

Biochemistry International
|April 1, 1988
PubMed
Summary

Sheep do not absorb creatine from their diet. Instead, sheep synthesize creatine, primarily in the liver, with skeletal muscle also contributing significantly to creatine metabolism.

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Area of Science:

  • Biochemistry
  • Physiology
  • Animal Metabolism

Background:

  • Creatine is vital for energy metabolism in muscle tissue.
  • Understanding creatine synthesis and utilization in livestock is crucial for nutritional and physiological studies.

Purpose of the Study:

  • To quantify creatine metabolism in sheep tissues.
  • To determine the sources and sinks of creatine and creatinine in the ovine body.

Main Methods:

  • Utilized multi-catheterized sheep to measure inflow and outflow of creatine and creatinine in various tissues.
  • Quantified renal clearance of creatine and creatinine.

Main Results:

  • Sheep do not derive creatine from the digestive tract.

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  • Total daily creatine synthesis is approximately 15 mmoles, with 80% occurring in the liver.
  • Hindlimb measurements suggest significant creatine synthesis in skeletal muscle.
  • Renal clearance of creatine and creatinine totals about 18 mmoles per day.
  • Metabolism of creatine in the heart, lung, and brain is minimal.

    • Conclusions:

    • Ovine creatine metabolism is primarily endogenous, with significant contributions from both liver and skeletal muscle.
    • The digestive tract is not a source of creatine for sheep.
    • Skeletal muscle plays a more substantial role in creatine synthesis than previously assumed.