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Analyzing Cell Surface Adhesion Remodeling in Response to Mechanical Tension Using Magnetic Beads
Published on: March 8, 2017
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Transient cell stiffening triggered by magnetic nanoparticle exposure
Jose E Perez1,2, Florian Fage1, David Pereira1
1Laboratoire Matière et Systèmes Complexes MSC, UMR 7057, CNRS & University of Paris, 75205, Paris Cedex 13, France.
Journal of Nanobiotechnology
|April 27, 2021
Summary
Magnetic nanoparticle uptake stiffens cells within minutes by altering cytoskeleton dynamics. This cell stiffening effect is transient, diminishing over hours and disappearing by 24 hours post-exposure.
Area of Science:
- Biophysics
- Nanotechnology
- Cell Biology
Background:
- Nanoparticle-cell interactions are crucial in nanomedicine.
- Cell mechanics significantly influence biological processes.
- The mechanical impact of magnetic nanoparticles on cells is understudied.
Purpose of the Study:
- Investigate how magnetic nanoparticles affect cell mechanical properties.
- Understand the time-dependent changes in cell mechanics following nanoparticle uptake.
Main Methods:
- Utilized a parallel plate rheometer to measure cell viscoelastic modulus.
- Monitored nanoparticle uptake and distribution using imaging.
- Assessed actin cytoskeleton remodeling in response to nanoparticles.
Main Results:
- Magnetic nanoparticle uptake caused rapid cell stiffening (<30 min) at the single-cell level.
- Cell stiffening was less pronounced in cell populations after 2h incubation.
- Actin bundling correlated with early stiffening, with effects normalizing over time.
Conclusions:
- Magnetic nanoparticle internalization and cytoskeleton remodeling transiently increase cell rigidity within minutes.
- The observed cell stiffening effect diminishes over hours and is absent at 24 hours post-internalization.
