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Label-Free Ratiometric Upconversion Nanoprobe for Spatiotemporal pH Mapping in Living Cells.

E Chen1, Ke Cai1, Xin Liu1

  • 1Key Laboratory of Phytochemical R&D of Hunan Province, and Chemical Biology & Traditional Chinese Medicine Research (Ministry of Education of China), Hunan Normal University Changsha 410081, China.

Analytical Chemistry
|April 27, 2021
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel pH sensor using self-assembled nanoparticles. This tool accurately tracks intracellular pH changes, aiding cell biology studies and disease diagnostics.

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Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Nanotechnology

Background:

  • Intracellular pH (pH) sensing is crucial for understanding cellular functions and diagnosing diseases.
  • Existing pH sensors often lack the sensitivity or specificity required for real-time intracellular measurements.

Purpose of the Study:

  • To engineer a novel, highly responsive pH sensor for intracellular applications.
  • To demonstrate the sensor's capability in monitoring dynamic pH changes within living cells.

Main Methods:

  • Fabrication of a pH sensor via one-step self-assembly of DSPE-PEG and phenol red on upconversion nanoparticles (UCNPs).
  • Characterization of the sensor's response to pH variations, including reversibility and speed.
  • In vivo imaging of spatial and temporal pH fluctuations during endocytosis and under external stimuli.

Main Results:

  • The developed sensor exhibited excellent reversibility and rapid response to pH changes.
  • The system successfully mapped spatial and temporal pH fluctuations within living cells.
  • The sensor effectively interrogated pH changes during cellular processes like endocytosis.

Conclusions:

  • The engineered phospholipid monolayer-based pH sensor offers a promising tool for real-time intracellular pH monitoring.
  • This technology has significant potential for advancing cell biology research and improving disease diagnostics.