Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR01:59

CRISPR

54.2K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
54.2K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

829
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
829

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Identification of the complete pathway for conversion of bilirubin to urobilinogen by human gut bacteria.

bioRxiv : the preprint server for biology·2026
Same author

Ecology and engineering to modify the bile acid output of a defined microbial community.

bioRxiv : the preprint server for biology·2026
Same author

A single-strain dropout screen reveals mechanistic links between microbial ecology and metabolism.

bioRxiv : the preprint server for biology·2026
Same author

Performance of IBD machine learning classifiers varies across microbiome training data independent of geographic diversity.

bioRxiv : the preprint server for biology·2026
Same author

Expanding the human proteome with microproteins and peptideins.

Nature·2026
Same author

<i>Blautia wexlerae</i> Transforms Dietary Fatty Acids to Activate Enteroendocrine Signaling and Improve Metabolic Health in Mice and Humans.

bioRxiv : the preprint server for biology·2026
Same journal

Non-canonical amino acid incorporation enables minimally disruptive labeling of stress granule and TDP-43 proteinopathy.

eLife·2026
Same journal

Analysis of dendritic input currents during place field dynamics.

eLife·2026
Same journal

TopoMetry systematically learns and evaluates the latent geometry of single-cell data.

eLife·2026
Same journal

Navigating the path: Advice to physician-scientists on choosing a clinical specialty.

eLife·2026
Same journal

Neural activity profiles reveal overlapping, intermingled subpopulations spanning area borders in mouse sensorimotor cortex.

eLife·2026
Same journal

The exquisite mechanics of a tsetse bite.

eLife·2026
See all related articles

Related Experiment Video

Updated: Nov 8, 2025

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models
13:47

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models

Published on: March 29, 2019

10.0K

CRISPR-based functional genomics in human dendritic cells.

Marco Jost1,2,3,4, Amy N Jacobson5,6, Jeffrey A Hussmann1,2,3,4,7

  • 1Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, United States.

Elife
|April 27, 2021
PubMed
Summary
This summary is machine-generated.

Researchers developed a CRISPR-Cas9 genome editing tool for human dendritic cells (DCs), enabling efficient gene knockouts. This method helps uncover how DCs control immune responses and identify factors influencing individual immunity.

Keywords:
CRISPRbacteroides thetaiotaomicrondendritic cellsfunctional genomicshumanimmunologyinfectious diseaseinflammationinter-individual variationmicrobiology

More Related Videos

CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery
07:49

CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery

Published on: May 30, 2025

1.8K
Identification of Functionally-Relevant Lentivirus Integration Sites in an Insertional Mutagenesis Cell Library
07:28

Identification of Functionally-Relevant Lentivirus Integration Sites in an Insertional Mutagenesis Cell Library

Published on: January 10, 2025

489

Related Experiment Videos

Last Updated: Nov 8, 2025

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models
13:47

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models

Published on: March 29, 2019

10.0K
CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery
07:49

CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery

Published on: May 30, 2025

1.8K
Identification of Functionally-Relevant Lentivirus Integration Sites in an Insertional Mutagenesis Cell Library
07:28

Identification of Functionally-Relevant Lentivirus Integration Sites in an Insertional Mutagenesis Cell Library

Published on: January 10, 2025

489

Area of Science:

  • Immunology
  • Genetics
  • Cell Biology

Background:

  • Dendritic cells (DCs) are crucial for immune responses, including antitumor and antiviral immunity, and host-microbe interactions.
  • Genetic manipulation of human DCs is challenging, hindering research into their specific immune functions.
  • Understanding DC mechanisms is vital for advancing immunotherapy and studying the host-microbiome interface.

Purpose of the Study:

  • To develop and validate a CRISPR-Cas9 genome editing method for human monocyte-derived DCs (moDCs).
  • To utilize this method for genetic screens to identify DC mechanisms regulating responses to microbial lipopolysaccharides.
  • To investigate donor-specific immune responses and identify genetic determinants of inter-individual variation.

Main Methods:

  • Development of a CRISPR-Cas9 genome editing system for human moDCs.
  • High-throughput genetic screening of over 300 genes in moDCs.
  • Analysis of DC responses to lipopolysaccharides from the human microbiome.
  • Assessment of donor-specific immune phenotypes and identification of candidate genes.

Main Results:

  • Achieved a median knockout efficiency of >94% across >300 genes in human moDCs.
  • Identified key mechanisms by which DCs modulate responses to microbial lipopolysaccharides.
  • Revealed significant donor-specific variations in lipopolysaccharide responses.
  • Discovered candidate genes responsible for inter-individual differences in DC immune function.

Conclusions:

  • The developed CRISPR-Cas9 method enables efficient genetic manipulation of human moDCs, facilitating functional studies.
  • This approach allows for the systematic dissection of immune signaling at the host-microbiome interface.
  • The findings pave the way for targeted DC engineering for applications like neoantigen vaccination and understanding personalized immunity.