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Nonlinear optical properties of potential sensitive styryl dyes.

J Y Huang1, A Lewis, L Loew

  • 1Department of Applied Physics, Cornell University, Ithaca, New York 14853.

Biophysical Journal
|May 1, 1988
PubMed
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This study details nonlinear optical dyes with large second harmonic signals, useful for detecting membrane potential changes. These dyes show potential for detecting signals from living cells.

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Area of Science:

  • Nonlinear optics
  • Biophysical chemistry
  • Materials science

Background:

  • Dyes that change optical properties with membrane potential are valuable tools in biophysics.
  • Second harmonic generation (SHG) is a sensitive nonlinear optical technique.

Purpose of the Study:

  • To characterize the nonlinear optical properties of novel dyes.
  • To assess the magnitude of second harmonic signals generated by these dyes.
  • To explore the potential of these dyes for detecting membrane potential in biological systems.

Main Methods:

  • Langmuir Blodgett (LB) monolayers were used to prepare thin films of the dyes.
  • Second harmonic generation (SHG) microscopy was employed to measure optical signals.
  • Structural analysis was inferred from SHG measurements.

Main Results:

  • The dyes exhibited exceptionally large second harmonic signals, among the largest detected to date.
  • SHG signals were sensitive to changes in the dyes' environment, correlating with membrane potential.
  • LB monolayers provided a suitable platform for characterizing dye properties.

Conclusions:

  • The characterized dyes possess significant nonlinear optical properties.
  • The large SHG signals suggest potential for sensitive detection of biological processes.
  • Further development could enable SHG-based imaging of membrane potential in living cells.