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Updated: Nov 7, 2025

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A Quantitative Glycomics and Proteomics Combined Purification Strategy
Published on: March 8, 2016
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One step carboxyl group isotopic labeling for quantitative analysis of intact N-glycopeptides by mass spectrometry
Zhenyu Sun1, Guanghui Ji2, Guoli Wang1
1Institutes of Biomedical Sciences and Shanghai Cancer Center, Fudan University, Shanghai 200032, China. ying@fudan.edu.cn luhaojie@fudan.edu.cn.
Abstract:
Here, we have developed an approach termed methylamine stable isotope labeling (MeSIL) to relatively quantify N-glycopeptides through one step labeling. It is the first time that this approach is applied to measure N-glycopeptide changes in huh7 cells after Zika virus infection and it has been revealed that differentially expressed N-glycopeptides played important roles in virus infection at the glycosylation site-specific level.

