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Updated: Nov 7, 2025

Membrane Remodeling of Giant Vesicles in Response to Localized Calcium Ion Gradients
Published on: July 16, 2018
Calcium-induced reversible assembly of phosphorylated amphiphile within lipid bilayer membranes
Yusuke Shimizu1, Kohei Sato1, Kazushi Kinbara2
1School of Life Science and Technology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan. koheisato@bio.titech.ac.jp kkinbara@bio.titech.ac.jp.
Abstract:
Inspired by calcium-induced reversible assembly and disassembly of membrane proteins found in nature, here we developed a phosphorylated amphiphile (PA) that contains an oligo(phenylene-ethynylene) unit as a hydrophobic unit and a phosphate ester group as a hydrophilic calcium-binding unit. We demonstrated that PA can assemble and disassemble in a reversible manner in response to the sequential addition of calcium chloride and ethylene-diaminetetraacetic acid within the lipid bilayer membranes for the first time as a synthetic molecule.
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