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Charge-reversal silver clusters for targeted bacterial killing.

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New silver nanoparticle clusters target bacteria effectively. Positively charged nanoparticles bind to negatively charged bacteria, showing high bactericidal ability and aiding skin wound healing in vivo.

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Area of Science:

  • Materials Science
  • Nanotechnology
  • Biomedical Engineering

Background:

  • Bacterial infections pose a significant global health threat.
  • Conventional antibacterial agents, including silver nanoparticles, lack targeted efficacy.
  • There is a critical need for efficient, low-toxicity, and targeted antibacterial agents.

Purpose of the Study:

  • To develop novel silver nanoclusters with tunable surface charges for targeted bacterial killing.
  • To investigate the charge-reversal properties of Ag(-)/Ag(+) nanoclusters in acidic environments.
  • To evaluate the in vitro and in vivo antibacterial efficacy of the developed nanoclusters.

Main Methods:

  • Synthesis of surface-charged silver nanoparticles (Ag(-) and Ag(+)) and their assembly into Ag(-)/Ag(+) clusters.
  • Control of zeta potential by adjusting the ratio of Ag(-) to Ag(+) nanoparticles.
  • Assessment of nanoparticle charge reversal in acidic conditions and subsequent changes in size.
  • In vitro evaluation of bactericidal activity against negatively charged bacteria.
  • In vivo study of skin wound healing in the presence of bacterial infection.

Main Results:

  • Ag(-)/Ag(+) nanoclusters exhibited controllable zeta potential based on the Ag(-) to Ag(+) ratio.
  • Acidic environments induced charge reversal in Ag(-) nanoparticles, leading to smaller, positively charged nanoparticles.
  • Positively charged nanoparticles demonstrated strong adsorption to negatively charged bacteria.
  • High in vitro bactericidal efficacy was observed.
  • In vivo experiments showed efficient antibacterial action and improved skin wound healing.

Conclusions:

  • The developed Ag(-)/Ag(+) nanoclusters offer a promising strategy for targeted antibacterial therapy.
  • The charge-reversal capability enhances their interaction with bacterial surfaces.
  • These nanoclusters represent an efficient antibacterial agent for combating bacterial infections and promoting wound healing.