Amino acid-functionalized carbon quantum dots for selective detection of Al³⁺ ions and fluorescence imaging in living cells

Carbon quantum dots (CQDs) are drawing tremendous attention due to their unique photoluminescence property and fascinating functions. Herein, we prepared novel CQDs functionalized with amino acids (AA-CQDs) by a one-pot hydrothermal method for selective detection of Al³⁺ ions and fluorescence imaging. The prepared AA-CQDs exhibit a novel triple-excitation and single-colour emission for fluorescent property. In addition, the AA-CQDs have a high absolute quantum yield (24.23%) and quantum lifetime (13.29 ns). Moreover, the AA-CQDs exhibit high selectivity and sensitivity for Al³⁺ by fluorescence enhancement. In pH 7.4 PBS solution, there was a good linear relation between the fluorescence intensity and the concentration of Al³⁺ in the range of 1-20 μmol L^-1; the limit of detection (3σ) was only 0.32 μmol L^-1. Furthermore, an AA-CQD probe was also utilized for detection of Al³⁺ in living cells based on excellent biocompatibility and endocytosis. Based on the concentration of Al³⁺ ions in cells and apoptosis data, there will be a quick reflect of apoptosis induced by aluminium ions via the fluorescence intensity of the AA-CQD probe. This work will set the stage for developing novel CQD-based biosensors in cell research.