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Related Experiment Videos

Lithium determined in serum with an ion-selective electrode.

R L Bertholf1, M G Savory, K H Winborne

  • 1Department of Pathology, University of Virginia Medical Center, Charlottesville 22980.

Clinical Chemistry
|July 1, 1988
PubMed
Summary

The lithium ion-selective electrode (ISE) accurately measures serum lithium levels in patients. This method demonstrates excellent linearity and precision, comparable to flame photometry and mass spectrometry.

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Area of Science:

  • Clinical Chemistry
  • Analytical Chemistry

Background:

  • Lithium is a crucial medication for treating bipolar disorder.
  • Accurate monitoring of serum lithium concentrations is essential for therapeutic efficacy and safety.

Purpose of the Study:

  • To evaluate the performance of a lithium ion-selective electrode (ISE) in the Du Pont Na/K/Li analyzer for serum lithium determination.
  • To compare the ISE method with flame photometry for lithium measurement.

Main Methods:

  • Serum lithium concentrations were measured in 106 patient samples using both ISE and flame photometry.
  • Linearity was assessed using serum and aqueous standards.
  • Precision was evaluated through within-run and day-to-day coefficients of variation (CVs).
  • Interference from common electrolytes and urinary lithium was investigated.

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Main Results:

  • The ISE method showed excellent agreement with flame photometry (slope = 1.004, SEE = 0.049 mmol/L).
  • Linearity was confirmed to >2.0 mmol/L.
  • Within-run CVs were 5.9% (low control) and 1.7% (high control); day-to-day CVs were 9.8% and 3.3%, respectively.
  • No significant interference was observed from sodium, potassium, calcium, magnesium, or urinary lithium.
  • Results aligned well with isotope dilution/mass spectrometry for survey materials.

Conclusions:

  • The lithium ISE in the Du Pont Na/K/Li analyzer provides accurate and reliable serum lithium measurements.
  • The method is suitable for routine clinical use, offering good precision and minimal interference.