Establishment and Development of a Quality Evaluation Method for Sangbaipi Decoction

Area of Science:

• Pharmacognosy
• Analytical Chemistry
• Traditional Chinese Medicine (TCM)

Background:

• Sangbaipi decoction (SD) is a traditional Chinese prescription with eight herbs.
• It is commonly used to treat asthma related to excess lung Qi and phlegm fire.

Purpose of the Study:

• To develop a comprehensive quality evaluation method for Sangbaipi decoction (SD).
• To ensure the quality and consistency of SD prepared from different batches.

Main Methods:

• High-Performance Liquid Chromatography (HPLC) fingerprint analysis was employed.
• Hierarchical Cluster Analysis (HCA) and Principal Component Analysis (PCA) were used for chemometric analysis.
• Ten batches of SD samples were analyzed to establish the quality control method.

Main Results:

• A total of 24 common peaks and six active components were identified in the HPLC fingerprint of SD.
• Hierarchical Cluster Analysis (HCA) and Principal Component Analysis (PCA) grouped the ten SD batches into four clusters.
• High similarity (≥0.934) was observed among the HPLC fingerprints of the ten batches, indicating good consistency.

Conclusions:

• HPLC fingerprints combined with chemometric analysis (PCA) and multicomponent determination provide a robust method for SD quality control.
• This study offers a foundation for enhanced quality evaluation, raw material selection, and preparation development of SD.
• The established method ensures the reliability and efficacy of this important TCM formula.