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Updated: Nov 7, 2025

Study of Short Peptide Adsorption on Solution Dispersed Inorganic Nanoparticles Using Depletion Method
Published on: April 11, 2020
Analysis of adsorption energy distribution in selected hydrophilic-interaction chromatography systems with amide,
Piotr Ziobrowski1, Marcin Chutkowski1, Mateusz Przywara1
1Department of Chemical and Process Engineering, Chemical Faculty, Rzeszów University of Technology, Rzeszów, Poland.
Abstract:
Adsorption mechanisms of caffeine, quercetin, and phenol as test substances in various chromatographic systems have been analyzed. The investigations were conducted using three different chromatographic columns packed with polar bonded stationary phases, that is, amide, amine, and zwitterionic. Methanol-water and acetonitrile-water systems with different organic solvent contents have been used as mobile phases. On the basis of adsorption isotherms obtained for the tested systems, Scatchard plots and adsorption energy distributions have been determined. The most likely retention mechanisms have been discussed. The results of investigations indicate that (i) the surfaces of tested adsorbents are energetically heterogeneous, and (ii) the main role in sorption mechanism is played by low-energy sites.
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