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Related Experiment Video

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An optimized protocol for establishing a chronic stress model in mice.

Chi Kin Ip1,2

  • 1Neuroscience Division, Garvan Institute of Medical Research, 384 Victoria Street, Darlinghurst, Sydney, NSW 2010, Australia.

STAR Protocols
|April 29, 2021
PubMed
Summary

This study presents a reliable chronic stress protocol for mice, addressing variability and unsuitability for female mice in existing methods. The new protocol ensures a consistent stress response across multiple mouse strains and sexes.

Keywords:
MetabolismModel OrganismsNeuroscience

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Area of Science:

  • Physiology
  • Animal Models
  • Stress Research

Background:

  • Chronic stress negatively impacts multiple organ systems and physiological functions.
  • Existing chronic stress models in mice exhibit significant response variability and are often unsuitable for female subjects.

Purpose of the Study:

  • To develop and present a standardized, reliable protocol for inducing chronic stress in mice.
  • To create a versatile model applicable to various physiological research settings.
  • To overcome limitations of existing protocols, particularly regarding female mice and response consistency.

Main Methods:

  • A detailed, step-by-step protocol for establishing a chronic stress model in mice.
  • Validation across multiple mouse strains including C57BL/6J, 129X1/SvJ, and B6.V-Lepob/J.
  • Testing conducted on both male and female mice to ensure sex inclusivity.

Main Results:

  • The protocol effectively produces a consistent and reliable response to chronic stress.
  • Demonstrated efficacy across diverse mouse strains.
  • Proven suitability for both male and female mice, addressing a key limitation in previous research.

Conclusions:

  • The developed protocol offers a robust and reproducible method for chronic stress modeling in mice.
  • This standardized approach enhances the reliability of stress-related research findings, particularly in studies involving female mice.
  • The protocol is adaptable for various physiological research applications.