Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

829
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
829
CRISPR01:59

CRISPR

54.2K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
54.2K
CRISPR and crRNAs02:53

CRISPR and crRNAs

18.0K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
18.0K
Homologous Recombination02:31

Homologous Recombination

58.3K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
58.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Transwell membrane material affects myogenic differentiation in human primary myoblast-adipocyte co-culture.

Physiological reports·2026
Same author

Importin-β1 functions as a chromatin sensor to position the contractile ring for cytokinesis.

Current biology : CB·2026
Same author

Benzylisoquinoline Alkaloid Production in Yeast via Norlaudanosoline Improves Titer, Selectivity, and Yield.

ACS synthetic biology·2026
Same author

Tri-Functional CRISPR Screen Reveals Overexpression of <i>QDR2</i> and <i>QDR3</i> Transporters Increase Fumaric Acid Production in <i>Kluyveromyces marxianus</i>.

ACS synthetic biology·2025
Same author

Open-space microfluidics as a tool to study signaling dynamics.

Lab on a chip·2025
Same author

Surface charge dictates the mechanism of cellular uptake of fluorescent amine passivated carbon dots.

RSC advances·2025

Related Experiment Video

Updated: Nov 7, 2025

Author Spotlight: Establishing CENP-E Knockout HeLa Cells &#8211; A Novel Approach to Study Kinesin-7 CENP-E Biology and its Inhibitors
11:49

Author Spotlight: Establishing CENP-E Knockout HeLa Cells – A Novel Approach to Study Kinesin-7 CENP-E Biology and its Inhibitors

Published on: June 23, 2023

958

CRISPR-Cas tools to study gene function in cytokinesis.

Mathieu C Husser1, Noha Skaik1, Vincent J J Martin1,2

  • 1Biology Department, Concordia University, MontrĂ©al, QC, H4B 1R6, Canada.

Journal of Cell Science
|April 29, 2021
PubMed
Summary
This summary is machine-generated.

Gene editing tools like CRISPR-Cas offer precise ways to study cell division (cytokinesis). This review explores their benefits for understanding protein function and gene expression in cell biology.

Keywords:
ActomyosinCRISPRCytokinesisGene editingRhoA

More Related Videos

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization
08:20

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization

Published on: September 2, 2021

4.3K
Using CRISPR/Cas9 Gene Editing to Investigate the Oncogenic Activity of Mutant Calreticulin in Cytokine Dependent Hematopoietic Cells
10:21

Using CRISPR/Cas9 Gene Editing to Investigate the Oncogenic Activity of Mutant Calreticulin in Cytokine Dependent Hematopoietic Cells

Published on: January 5, 2018

13.4K

Related Experiment Videos

Last Updated: Nov 7, 2025

Author Spotlight: Establishing CENP-E Knockout HeLa Cells &#8211; A Novel Approach to Study Kinesin-7 CENP-E Biology and its Inhibitors
11:49

Author Spotlight: Establishing CENP-E Knockout HeLa Cells – A Novel Approach to Study Kinesin-7 CENP-E Biology and its Inhibitors

Published on: June 23, 2023

958
A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization
08:20

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization

Published on: September 2, 2021

4.3K
Using CRISPR/Cas9 Gene Editing to Investigate the Oncogenic Activity of Mutant Calreticulin in Cytokine Dependent Hematopoietic Cells
10:21

Using CRISPR/Cas9 Gene Editing to Investigate the Oncogenic Activity of Mutant Calreticulin in Cytokine Dependent Hematopoietic Cells

Published on: January 5, 2018

13.4K

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • Cytokinesis, the final stage of cell division, is crucial for organism development.
  • Traditional overexpression studies limit our understanding of essential protein functions during cytokinesis.
  • Gene editing offers a powerful alternative to study protein function in a physiological context.

Purpose of the Study:

  • To review the advantages of gene editing technologies for studying cytokinesis.
  • To highlight the application of CRISPR-Cas systems in understanding cytokinesis protein function.
  • To outline methodologies for gene editing and precise gene expression control in cell division research.

Main Methods:

  • Review of existing literature on gene editing applications in cell biology.
  • Discussion of CRISPR-Cas, CRISPR interference (CRISPRi), and CRISPR activation (CRISPRa) techniques.
  • Exploration of gene editing for creating functional mutations and fluorescent probes.

Main Results:

  • Gene editing, particularly CRISPR-Cas, enables precise functional studies of cytokinesis proteins.
  • CRISPRi and CRISPRa allow for controlled manipulation of gene expression to investigate protein roles.
  • Gene editing facilitates the study of essential genes and protein functions in relevant cellular contexts.

Conclusions:

  • Gene editing provides a robust platform for advancing cytokinesis research.
  • The application of gene editing tools is essential for a deeper understanding of cell division mechanisms.
  • This review serves as a guide for utilizing gene editing in studying cytokinesis and other cellular processes.