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Differences between serum and urinary LH in hypergonadotropic states.

M F Silva de Sá1, M J Matthews, R W Rebar

  • 1Department of Reproductive Medicine, School of Medicine, University of California, San Diego, La Jolla 920993.

European Journal of Obstetrics, Gynecology, and Reproductive Biology
|May 1, 1988
PubMed
Summary
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Investigating luteinizing hormone (LH) forms, this study found differences in immunoreactivity and receptor binding between serum and urine. These molecular changes suggest alterations during LH metabolism and excretion.

Area of Science:

  • Endocrinology
  • Molecular Biology
  • Biochemistry

Background:

  • Luteinizing hormone (LH) plays a crucial role in reproductive function.
  • Understanding the molecular forms of LH is essential for interpreting immunoassays and biological activity measurements.

Purpose of the Study:

  • To investigate the relationship between molecular configuration, immunoreactivity, radioligand binding, and biological activity of LH.
  • To compare the elution profiles of immunoreactive and radioligand receptor-active LH in serum and urine.

Main Methods:

  • Gel filtration chromatography (Sephadex G-100) was used to separate LH forms.
  • Samples from normal cycling, postmenopausal, and castrate women were analyzed.
  • Immunoreactivity, radioligand binding, and bioactivity (rat interstitial cell-testosterone assay) were measured.

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Main Results:

  • A major peak of LH immunoreactivity was observed in both serum and urine, but urinary immunoreactivity eluted later than serum.
  • Radioligand receptor activity for LH consistently peaked in the same fractions as serum immunoreactivity.
  • Bioactivity correlated well with receptor activity, but not consistently with immunoreactivity.

Conclusions:

  • Data suggest alterations in the molecular form of LH during metabolism and/or excretion.
  • Differences in peptide structure or carbohydrate moieties may account for observed variations in LH forms.
  • Discrepancies between immunoreactivity and bioactivity highlight the complexity of LH analysis.