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Related Concept Videos

CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR/Cas9 Genome Editing01:28

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR and crRNAs02:53

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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What is Genetic Engineering?00:49

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Overview
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Homologous Recombination02:31

Homologous Recombination

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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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Related Experiment Video

Updated: Nov 7, 2025

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization
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A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization

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Using CRISPR to understand and manipulate gene regulation.

Ersin Akinci1,2, Marisa C Hamilton1, Benyapa Khowpinitchai1

  • 1Division of Genetics, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA 02115, USA.

Development (Cambridge, England)
|April 29, 2021
PubMed
Summary

CRISPR-Cas9 technology precisely manipulates gene regulation, advancing developmental biology. This powerful tool enhances understanding of gene regulatory networks and cell fate, promising new disease treatments.

Keywords:
CRISPR screeningCRISPR-Cas9Disease modelingEpigeneticsGene regulation

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Area of Science:

  • Developmental Biology
  • Genetics
  • Molecular Biology

Background:

  • Gene expression in specific cell types is crucial for development.
  • Traditional gene regulation studies relied on observational methods lacking precision.
  • CRISPR-Cas9 offers precise genomic manipulation for studying gene regulation.

Purpose of the Study:

  • To explore the application of CRISPR-Cas9 technology in gene regulation research.
  • To discuss the potential of CRISPR-based tools in understanding the gene regulatory code.
  • To highlight CRISPR's role in manipulating cell fate and treating genetic diseases.

Main Methods:

  • CRISPR-Cas9 for precise manipulation of genomic sequences.
  • CRISPR for epigenetic functionalization and gene expression studies.
  • Development of new CRISPR-based tools and methods.

Main Results:

  • CRISPR-Cas9 has enabled precise manipulation of gene expression.
  • New insights into gene regulatory mechanisms have been discovered.
  • CRISPR technology is transforming the study of gene regulation.

Conclusions:

  • CRISPR-Cas9 toolbox is revolutionizing gene regulation research.
  • Future applications include predicting gene regulatory networks and cell fate reprogramming.
  • CRISPR holds promise for disease modeling and treating genetic disorders.