Bio-orthogonal chemistry enables solid phase synthesis and HPLC and gel-free purification of long RNA oligonucleotides
Muhan He1, Xunshen Wu1, Song Mao1
1University at Albany, Department of Chemistry, Albany, NY,12222, USA. mroyzen@albany.edu.
Abstract:
Solid phase synthesis of RNA oligonucleotides which are over 100-nt in length remains challenging due to the complexity of purification of the target strand from failure sequences. This work describes a non-chromatographic strategy that will enable routine solid phase synthesis of long RNA strands.
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