Related Experiment Video
Updated: Nov 7, 2025

07:42
Quasistatic Mechanical Testing for Computer-Aided Design and Manufacturing Occlusal Veneers Cemented to Milled Dentin Analog Material
Published on: December 20, 2024
558
Tooth Discoloration Using Calcium Silicate-Based Cements For Simulated Revascularization in Vitro.
Rui Barbosa Marques Junior1, Kusai Baroudi1, Alison Flávio Campos Dos Santos1
1Postgraduate Program in Dentistry, School of Dentistry, University of Taubaté, Taubaté, Brazil.
Brazilian Dental Journal
|April 29, 2021
Summary
Endodontic revascularization can cause tooth discoloration. Calcium silicate cements with bismuth oxide showed the highest color change, while those with calcium tungstate and zirconium oxide had less discoloration, making them better alternatives.
Area of Science:
- Endodontics
- Dental Materials Science
- Biomaterials
Background:
- Endodontic revascularization is a promising treatment for immature necrotic teeth.
- Tooth discoloration is a common complication following endodontic revascularization procedures.
Purpose of the Study:
- To evaluate the color alteration of extracted premolars after simulated endodontic revascularization using calcium silicate-based cements with different radiopacifying agents.
- To compare the discoloration potential of cements containing bismuth oxide, calcium tungstate, and zirconium oxide.
Main Methods:
- Extracted premolars were prepared and filled with blood to simulate revascularization.
- Three calcium silicate-based cements (CSBi, CSW, CSZr) and a control (TFZn) were applied.
- Color change (ΔE) was measured using spectrophotometry over five months.
Main Results:
- All tested calcium silicate-based cements caused clinically perceptible tooth discoloration.
- The cement with bismuth oxide (CSBi) exhibited the highest color alteration (ΔE).
- Cements with calcium tungstate (CSW) and zirconium oxide (CSZr) showed similar, intermediate ΔE values, with CSBi causing the greatest decrease in yellowness.
Conclusions:
- Bismuth oxide as a radiopacifier leads to significant tooth discoloration in endodontic revascularization.
- Calcium tungstate and zirconium oxide are viable alternatives to minimize tooth discoloration.
- These findings suggest that material selection is crucial for aesthetic outcomes in endodontic revascularization.

